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Training schedule of the subject
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| TOPIC
| Classes volume (hours)
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| Total
| Lectures
| Lab.
classes
| Self study
| Individual work
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| | module № 1 “Optical methods for biotechnological industries monitoring”
| | 1.1
| Introduction. Methods for biotechnological industries control. Physico-chemical methods. The general theory of light absorption.
Spectroscopy and electromagnetic radiation. Instruments for measuring light absorption.
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| | 1.2
| Optical inspection methods based on photon emission and absorption of light by a substance. The use of optical methods of control. Spectroscopic methods and instruments.
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| | 1.3
| Methods of optical control based on the laws of refraction of light, optical rotation.
Refractometry, polarimetry. Types of refractometers, polarimeters and work with them..
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| | 1.4
| Quality control components and nutrient media control of dispersion by optical methods.
Preparation of culture media components to quality control. Diffractometry. Devices and work with them.
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| | 1.5
| Module control paper №1
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| -
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| | Total for module № 1
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| | module №2 “Physical - chemical methods for air and water monitoring at the biotechnological industries”
| | 2.1
| Chromatographic methods of analysis in biotechnology.
Gas and thin layer chromatography. Thin layer chromatography. Preparation of samples for chromatographic methods. Gas chromatography. Types of gas chromatograph..
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| | 2.2
| Water Analysis, routine analysis and special techniques. Liquid chromatography. Preparation of samples for liquid chromatography analysis. Types liquid chromatograph. Special methods.
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| | 2.3
| X-ray spectroscopy, electron microscopes, microanalysis
X-ray fluorescence methods, imaging, spectroscopy electrons.
Types of electron microscopes. Preparation of the specimens for research in the electron microscope.
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| | 2.4
| Spectroscopy of mass. Analysis of proteins secondary-ion mass spectrometers isotopic analysis. Preparation of the specimens for research. Analysis of air. Devices for the analysis of gas composition of air.
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| | 2.5
| Module control paper №2
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| -
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| | Total for module № 2
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| | module № 3 “Term paper”
| | 3.1
| Term paper
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| -
| -
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| | Total for module № 3
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| -
| -
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| | Total for semester
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| | Total for the subject
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Development of the didactic process for different types of classes
Lectures, their subject matters and planned hours
| №
| TOPIC
| Academic Hours
| |
|
| Lectures
| Self study
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|
|
| | module № 1 “Optical methods for biotechnological industries monitoring”
| | 1.1
| Introduction. Physico-chemical methods.
The general theory of light absorption by molecules.
On-line and off-line methods
Features of methods and devices for industrial control and research laboratories.
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| | 1.2
| Spectroscopy and electromagnetic radiation. Instruments.
Spectrophotometers, design principles.
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| | 1.3
| Methods based on photon emission and absorption of light by a substance...
Practical use of spectrophotometers. IR, UV and visible - features of application and design of devices.
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| | 1.4
| The use of optical methods of control.
Spectroscopic methods and instruments.
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| | 1.5
| Methods based on the laws of light refraction, optical rotation. Analysis of carbohydrates in the laboratory and at the outdoor conditions.
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| | 1.6
| Refractometry, polarimetry.
The index of refraction, methods of measurement devices.
Refractometer, saccharimeter. Tables of refraction index values according to the concentration of sugar in aqueous solution.
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| | 1.7
| Diffractometry. Instruments
Detectors for spectroscopy. CCD, photodetectors, photomultiplier tube.
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| | 1.8
| Module control paper №1
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| | Total for module № 1
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| | module №2 “Physical - chemical methods for air and water monitoring at the biotechnological industries”
| | 2.1
| Chromatographic methods of analysis in biotechnology. Classification of chromatography methods.
Features and disadvantages of chromatography.
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| | 2.2
| Preparation of samples for chromatographic methods.
Methods of extraction solvent selection; cleaning of the sample.
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| | 2.3
| Gas chromatography.
Modern devices, application examples of gas chromatography.
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| | 2.4
| Water Analysis, routine analysis and special techniques.
Liquid chromatography.
Practical application of "HPLC" in industrial and research laboratories.
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| | 2.5
| Microanalysis. Types of electron microscopes.
Programs for computer simulation of the electron microscope and X-ray spectroscopy "Montecarlo" method for simulation the motion of particles in the environment.
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| | 2.6
| X-ray methods of research, Spectroscopy, fluorescence emission of photoelectrons. Тomography.
X-ray spectrometers crystal-diffraction and semiconductor.
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| | 2.7
| Spectroscopy of masses. Analysis of proteins, gases and air; the isotopic analysis Special methods for mass analysis of the macromolecules;
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| | 2.8
| Secondary-ion mass spectrometers,. Preparation of the specimens. Instruments.
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| | 2.9
| Module control paper №2
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| | Total for module № 2
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| | Total for the subject
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Laboratory classes, their subject matters and planned hours
| №
| TOPIC
| Academic Hours
| | Laboratory classe
| Self study
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| | module № 1 “Optical methods for biotechnological industries monitoring”
| | 1.1
| Selection of the operating wavelength for photometric analysis.
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| | 1.2
| Using of photometry to determine the concentration of substances in solution.
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| | 1.3
| Determination of solution concentration by the refractometric method.
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| | 1.4
| Impurities detecting in solution via refractometry and polarimetry.
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| | Total for module № 1
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| | module №2 “Physico-chemical methods for air and water monitoring at the biotechnological industries”
| | 2.1.
| Chromatographic methods of separation and analysis.
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| | 2.2.
| Electron microscopy and X-ray microanalysis. Computer simulation.
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| | 2.3.
| Definition of agar quality.
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| | 2.4.
| Determination of natural and pipe-water quality
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| | 2.5.
| Determination of purified water quality.
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| | Total for module № 2
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| | Total for the subject
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Individual work
| №
| TOPIC
| Classes volume (hours)
| |
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| |
| Fundamentals of spectroscopy. Spectroscopy of waves and particles. Detectors.
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| |
| Methods of optical monitoring based on the absorption and emissions of light and particles.
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| Methods of optical monitoring techniques based on the laws of light refraction. Diffraction. Optical rotation.
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| |
| Electronic and ionic projectors nuclear probe X-ray spectroscopy..
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| |
| Methodology of spectral imaging, chemical maps. Micro- and nano-tomography.
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| | Total for the subject
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2.2.4.
Student self-study, its content and planned hours
| №
| Self-study Content
| Academic Hours
| |
| Work with lecture material
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| | | Preparing for laboratory classes
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| | | Preparing for module testing
|
| | | Term paper
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| | Total for the subject
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Term paper
Term paper on the subject is carried out in the third semester, in accordance to the course guidelines, in order to consolidate and deepen the theoretical knowledge and skills acquired by students during the subject learning.
Term paper performance is an important step in preparing for thesis work implementation by future specialist biotechnologist.
For the successful term paper performance the student should know the theoretical background on the topic of the term paper, the basic techniques of experimental research, be able to analyze and organize literature data, formulate object, subject, purpose and tasks of the term paper according to the chosen theme
The time required for term paper performance is up to 36 hours of self work.
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